![]() ![]() FlowJo will convert the image file to an FCS file, and should appear in the Finder/ Explorer window as well as the Workspace. Select the image file (JPEG, GIF, BMP, or PNG) and drag it to the workspace.Ĥ. Open the Finder or Explorer window to find the image file of interest.ģ. Open a new workspace in FlowJo v10 (10.0.7 or later).Ģ. The following steps will guide you through opening an Image file in FlowJo v10.ġ. ![]() ImageJ, Adobe Photoshop, Macintosh’s Preview App, etc). Fortunately, there is a plethora of image software available to interconvert almost any file type (e.g. TIFF, PDF, and other image file types must first be converted to an accepted format before opening them in the FlowJo workspace. ![]() Many laboratory instruments will save imaging data as a proprietary file type, or as TIFFs. ![]() Accepted Image file typesĬurrently FlowJo v10 will open JPEG, PNG, BMP, and GIF images. This is particularly useful for high throughput applications or when you have several images from an experiment and need to quickly pinpoint which files contain images and have a pixel hue or saturation above a specified threshold. images that have high expression of a particular tag, antigen, or protein). That is, grouping images based on specific characteristics (e.g. GFP+ areas), MFI’s (median fluorescence intensity) and other statistics can be added, and reports can be generated via the Table and Layout Editors to display results across samples or experiments.īeyond analysis of individual image files, FlowJo v10’s image analysis power can be applied to meta-analysis. Similar to events or cells in an FCS file, pixels are characterized by their color and brightness attributes. In this case pixels are treated like events gates can be created on regions of interest (e.g. Image files, like FCS files, can be parsed into component parts for analysis. Working with images? You can open, gate, and add statistics to image files with the latest release of FlowJo v10 (10.0.7 or later). ![]()
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